St. Cercet. Virusol. 37 (2) 2007

Contents

• 67 - Corelation between histopatological aspects and biochimical
  markers used for monitorisation of Hcv infection under treatment
  Camelia Sultana, Loredana Manolescu, Simona Ruţă

  “C. Davila” University of Medicine, “Åžtefan S. Nicolau” Institute of Virology, Bucharest, Romania

  [more...]

  Abstract. Histopatological stadies of hepatic lesions represent the objective factor of chronic hepatitis prognossis and are very important in initiasion and monitorisation of the treatment of hepatitis C. The biopsy–punction is an invasive method, with several adverse reactions, while biochemical markers are easy to determine and risk freee investigations for the patient. Our aim was to corelate biochemical constant with the severity of hepatic lesions.Methods. Histopatological studies were apreciated uppon METAVIR clasification in 33 patients diagnosed in the Institute of Virology with chronical C hepatitis fibrossis. Results. Acording with the severity of hepatic impairment, the patients were divided in 3 groups: (a) without important histological lesions–57,6%; (b) with important histological lesions –27,3%; (c) advanced lesions with several fibrotic septs or chirossis –15,1%. Hepatic fibrosis and the necro-inflamatory activity clinical important (F2, F3, F4 ÅŸi respectively, A2 or A3) are diagnosed better with the logistic regresion, according to an index that combines 6 biochemical constants.
  Conclusions. The fibrosis index can be a noninvasive method, easy to use when evaluating and treating this infection.

  Keywords: chronic hepatitis C, histopatological grades, biochimical markers, fibrosis index.

• 75 - Modulation of nk cell function by activatory and inhibitory membrane receptors

  Cecilia Galatiuc, Mirela Hirt, Marinela Bostann

  Immunology Center - Institute of Virology “Åžtefan S. Nicolau”, Bucharest, Romania.

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  Abstract. NK cells are auto-regulated effectors of natural immunity, their reactions depending on the nature, sequence, and intensity of external stimuli received via their membrane receptors.In vitro and in vivo studies regarding the modulation of NK cells revealed a variety of activators (i.e. cytokines IFN and IL-2) and inhibitors [soluble agents acting at the level of recognition and binding to target cells (EDTA, cyclophosphamide, corticosteroids) or NK on cell-mediated lysis (cyclic nucleotides, prostaglandins, TIFF- etc.]. An important down modulation of NK cell function occurred post interaction of the immunoglobulin molecules from IgG, IgA and IgM classes with their specific membrane Fc receptor (FcR) counterparts. conflictive evidences accumulated about the the coexistence of two FcRs (CD16 and CD32) reacting with IgG monomers. Moreover, the dichotomy of FcR IIIA post binding intracellular signals delivered (inducing NK activation after ligation of anti-CD16 monoclonal s of IgG1 isotype, and drastic suppression with cytophilic IgG monomers) the importance of in vivo pre-activation state of the NK cells, imposed supplemental in vitro studies aimed to establish the mechanisms of signal hierarchy. Recent literature data regarding some particular unorthodox regulatory mechanisms of NKR mediated regulation of NK cell functions brought some light in our understanding of the complexity of NK cell responses.

  Keywords: NK, ADCC, RFc, RNK, KIR.

• 85 - Database for the information management in the genital infection with papilloma viruses

  C.N. Zaharia, A.M. Popovici, Gabriela Anton

  „Åžtefan S. Nicolau” Institute of Virology, Bucharest, Romania

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  Abstract. For the management of information contained in the medical case records, as well as of the results of virological experimental investigations in their dynamics over time, a database was created in Microsoft Access 2003. In the case of researches regarding the genital infection with human papilloma viruses, the database is useful in the standardization of the clinical observations registration and in the correlation with the results of the virological investigations. The database represents the informational support available for the correct management of patients, by the corroboration results of clinical and paraclincal investigations.

  Keywords: medical information processing with dynamic over time, cervical cancer, human papilloma virus.

• 91 - Shipping clinical samples

  Dragomir Laura Georgiana

  “Åžtefan S. Nicolau” Institute of Virology, Bucharest, Romania

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  Abstract. Every day clinicians, laboratory researchers, and technicians send and/or receive specimens, infectious agents and other biological materials. Each of these individuals is responsible for complying with all the applicable regulations. This includes: properly packaging, labeling and marking the shipment, accurately completing the paperwork, making advance arrangements as needed with the recipient and the carrier, obtaining any permits needed to import or export biological materials.
  Anyone shipping or receiving potentially hazardous materials such as: infectious substances, diagnostic specimens, genetically modified microorganisms, potentially hazardous biological materials, and/or dry ice, is required to have International Air Transport Association (IATA) and Department of Transportation (DOT) training in the general requirements of the regulations. The training must be repeated every two years as long as you ship or receive such materials.

  Keywords: transport conditions, IATA, infectious substances.

• 99

  Abstracts – Åžtefan S. Nicolau Conference