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St. Cercet. Virusol. 38 (2) 2008

Contents

 

 

53 - Comparative study regading the sensitivity of an in – house  technique versus commercial kit innolipa in HPV – DNA detection
Irina Huica1, Iuliana V. Iancu, Zorela Sgarbura2, Dominic Iliescu, Demetra Socolov, Gabriela Anton1 
1Stefan S. Nicolau Institute of Virology, 2Filantropia Hospital, Bucharest, Romania, 3UM
F Craiova, Romania, 4UMF Iasi, Romania
 Abstract. Due to their involvment in the etiology of cervical cancer, identification of human papillomaviruses (HPV) DNA associated with the cytological screening plays an important role in the management of HPV related genital infections. For this matter the viral testing method is important for the identification and surveillance of the patients which exhibit a high risk of cervical neoplasia development. Taking into account that only a small percentage of subclinical infections could be diagnosed by cytological/colposcopical examination, performing a viral test on a larger number of subjects is necessary. Detection of HPV genital infections is based exclusively on molecular biology techniques which highlights viral DNA. Our objective was to test the sensibility of a PCR in-house technique versus a commercial kit.
 
Keywords:HPV, PCR GP5+/6+, InnoLipa.
 
59 - The prevalence of hpv genotypes in unprotected sex
Viviana Dragodan, Constanta Antipa, Mihai Stoian
Stefan S. Nicolau Institute of Virology, Bucharest, Romania
Abstract. One unprotected sexual contact can facilitate contracting of one or more sexually transmitted infections. Sexual transmission (vaginal, oral, and anal) of HPV is well known. In the first 10-20 years follow the onset of sexual life, the risk of HPV infection through unprotected sexual contacts is very high. The objective of this study was to establish the HPV genotypes prevalence in unprotected sexual contact transmission. Study group was comprise from 46 patients with HPV infection presumptive diagnosis, which were tested in order to detect and genotype HPV DNA. HPV DNA was detected in 33 (71.74%) of all tested cases. Most encountered HPV genotypes were those from high risk group: HPV31 (24.24%), HPV16 (18.18%), HPV18 (9.09%) and HPV51 (9.09% ), followed by those with low risk: HPV6 (24.24%). The frequency of certain HPV genotypes acquired through unprotected sexual contacts worth evaluated, especially in the case of latent asymptomatic infections, along with hormones and immune factors local.
 
Keywords: human papilloma virus (HPV), genotype, sexually transmitted diseases (STD).
 
65 - Molecular interactions identification method based on optical amplification of liquid crystals disturbance
C.N. Zaharia1, Mihaiela Albulescu1, Alexandrina Nuta2, Alexandra Sorescu2
1Stefan S. Nicolau Institute of Virology, Bucharest, Romania, 2National Research & Development Institute for Chemistry & Petrochemistry – ICECHIM, Bucharest, Romania
Abstract. The paper presents some aspects regarding detection of molecular interactions based on systems withcontroled topography. The method is illustrated by antigene-antibody interaction type. The procedure can be used to develop viewing technics, through liquid crystals, for specific interactions antigene - antibody type. Thismethod has high sensibility and specificity, and represents a choice for viral agents identification current technics.
 
Keywords:antigene - antibody, SAM, liquid crystals, optical amplification, biosensors.
 
73 - Clinical research – from principles of ethics to legal accountability in clinical trials on human subjects
Andrei Nanu
 Medright Experts, str. Alexandru Constantinescu nr. 30, sector 1, Bucharest, Romania  
Abstract. The clinical research has two major components: the science and the medical ethics. The developments in the health sciences created concerns about the capacity of the traditional medical ethics to provide moral guidelines adapted to the new environment and determined the development of biomedical ethics. The goal of the bioethics is to create standards of conduct for institAbstract. de la utions and practices of medicine, as an effort to protect the rights of the patients and research subjects. The biomedical ethics principles are echoed and referred in the legislation regarding the clinical research. In order to obtain a consensus regarding the good practice in clinical trials to reach, an International Conference on Harmonisation (ICH) was called into being. ICH provide a unified standard regarding the good clinical practice (GCP). Based on these standards, the European Union issued directives for the Member States, including Romania relating to the implementation of the instructions for good clinical practice in the conduct of clinical trials in the national legislations.  
 
Keywords: biomedical ethics, clinical trials, legislation.
 
 
79 - VIRCELL Company (Spain)
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