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The role of E6/E7 HPV16 oncogenes in chromatin remodelling through components of NuRD complex (MBD2, MBD3)

Project Code: PN-II-RU-TE-2014-4-2502


Concrete objectives of the project

The study of HPV carcinogenic mechanisms continues to attract interest especially regarding the molecular biology of cervical cancer, in order to find the most appropriate cellular or viral targets in this disease. All the research in this field aims to discover new approaches for prophylactic and therapeutic purposes in the benefit of patients. The project aims to analyze MBD2 and MBD3 genome-wide binding pattern in the context of the E6/E7 oncogene activity.
 For this purpose, the specific objectives of the proposal are as follows:
-    HPV 16 E6/E7 oncogene silencing in SiHa cell culture (as experimental model);
-    Detection of MBD2, MBD3 genome wide DNA binding pattern (e.g. promoters, gene control region, transcriptional enhancers, etc.) in untreated and siRNA treated SiHa cell culture;
-    Comparative study between the MBD2 and MBD3 genome wide DNA binding pattern;
-    Cellular targets data integration in viral infection context.