St. Cercet. Virusol. 38 (1) 2008

3 - The role of Chlamydia trachomatis infection in oligoasthenospermia

Viviana Dragodan, Constanţa Antipa, Mihai Stoian

Ştefan S. Nicolau Institutul of Virology, Bucharest, Romania

Abstract. Oligoasthenospermia represintes one of the most important causes of masculin infertility. Recentes studies show that one of five patients with infertility have antecedents of sexual transmitted diseases (Chlamydia trachomatis, Neisseria gonorrhoeae, Ureaplasma urealyticum, Mycoplasma genitalium etc.). The target of this study is to investigate the implication of Chlamydia trachomatis in oligoasthenospermia. The group of study was formed by 31 patients with diagnosis of oligoasthenospermia, couple infertility, leucocytospermy and they were tested to evidence the infection with Chlamydia trachomatis. The antigen LPS of Chlamydia trachomatis was found at one patient (3,22%) and the serological evaluation of Chlamydia trachomatis showed that the antibodies anti Chlamydia trachomatis IgG was founded at 5 patients (22,72%), IgA at one patient (4,54%) and IgG + IgA at 2 patients (9,09%) of all the group. The role of Chlamydia trachomatis in oligoasthenospermia is in course of evaluation, despite of the fact that some authors considere it relevant for masculine infertility beside of neurohormonal, genetics, immunologicals and viral factors.

Keywords: Chlamydia trachomatis, antigen LPS of Chlamydia trachomatis, oligoasthenospermia.

9 - Microglia as innate immunity effector components cells in central nervous system

Daniela Pasarica¹, Mihaela Gheorghiu¹, T. Trandafir¹, M. Stoian²

¹U.M.F. Carol Davila Bucharest, Catedra de fiziopatologie si imunologie,²Stefan S. Nicolau Institutul of Virology, Bucharest, Romania

Abstract. Microglia are innate immune cells in central nervous system (CNS) that have been traditionaly regarded as CNS macrophages-like cells. These cells are myeloid origin and take up residence in the CNS during embryogenesis. Following activated microglia to a consequence of a CNS injury, stroke in response to invading viruses or in neurodegenerative diseases, they play important roles in innate and apaptative immune reaponses. As immune cells they have a high mobility and a double responces characteized by enhanced phagocytosis and pro-inflammatory cytokine production as well as adaptative activation of T cells (APC function) (1). Microgliamay also participate or contribute to degenerativeevents underlying neurological disorders, including Alzheimer's, Parkinson's,Huntington's Diseases, multiple sclerosis and inhibition of microglia functions contributes to benefit effect and could be key in turning a pathogenic into a therapeutic cells.

Keywords: innate immunity, microglia, central nervous system, pro-inflammatory cytokines, Toll like receptor

17 - Prognostic biomarkers in chronic lymphocytic leukemia

Claudia Maglas, D. Hotnog, Lorelei I. Brasoveanu, Viviana Roman

Center of immunology, Stefan S. Nicolau Institutul of Virology,Bucharest, Romania

Abstract. The individual prognosis of patients with chronic lymphocytic leukemia (LLC) is extremely variable. Although these criteria remain the basis for assessing prognosis in CLL, particularly the clinical stages of the disease, icannot be performed an acurate evaluation. During the last years many efforts have been made to identify prognostic biomarkers in LLC, being proposed and studied a large number of biological factors. A number of this biological markers, particulary serum biomarkers, cytogenetic abnormalities, CD38, ZAP-70 expression or IgVH mutations offer important, independent prognostic information. In this review it is mentioned the advantages and drawbacks of these biomarkers, therefore the necessity of continuing the studies in this direction.

Keywords: chronic lymphocytic leukemia (LLC), biomarkers, ZAP-70, CD38, IgVH.

25 - Biosecurity and biosafety management in biological research laboratories environment

C.N. Zaharia, N. Steiner

Stefan S. Nicolau Institutul of Virology, Bucharest, Romania

Abstract. The laboratories that have biosafety programs fulfill much of the base requirements for biological materials safety. For the laboratories that did not handle selectionated agents, the access control and the requirements of training indicated for the laboratories with biosafety levels 2 and 3 could provide enough safety for the materials that are studied. The security evaluations and the additional measures must be considered when the selectionated biological agents, or other agents, that interests in special the public health or some of the agricultural authorities, for use in human or animal vaccine preparation. The term of “biosecurity” has multiple definitions. In the food industry, this term refers to the animal protection from microbiological or viral contamination. In some countries the term of biosecurity is used as replacement of the term biosafety. In this work the term of biosafety is distinguished from the term of biosecurity, the last term being used in actions and measures for the loss, theft, diversion or deliberate use of the microbiological agents.

Keywords: biological materials, biological agents, occupational health.

31 - 4th Acad. Nicolae Cajal Symposium - Abstracts

St. Cercet. Virusol. 38 (1) 2008

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